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The Endpoint Chromogenic Limulus Amebocyte Lysate test was used to quantify the remaining endotoxin in the target solution. Briefly, Limulus Amebocyte Lysate was incubated with the hGCSF sample at 37uC for 10 min before the substrate was added. Stop agent was then added to the mixture and the released p-nitroaniline was evaluated by photometric measurement at 405410 nm. added to each well and the
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alysis A non-linear regression analysis was used to determine the MNFS-60 cell proliferation dose-response to hGCSF. The data were fitted using the following equation and Microsoft Excel software, where Re is response of the cells, Bl is the baseline at low concentration, Max is the maximum response, conc is the concentration of the protein, and Hs is the Hill coefficient of stimulation, Bh is th
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Food safe-keeping is actually especially a good essential part of success.
As vegetables especially meat have to be kept in situation they must become used inside the near future.
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